Both HDL3 and HDL2 Exert a Powerful Anti-Oxidative and Protective Effect against Acceleration of Oxidative Modification of LDL by Ascorbic Acid

• SAKUMA Nagahiko

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• SAEKI Tomoaki

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• YAJIMA Kazuhiro

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• HIBINO Takeshi

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• YOSHIDA Takayuki

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• MIZUNO Hiromi

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• MUKAI Seiji

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• SAKATA Seiichiro

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

• KUNIMATSU Mitoshi

  Department of Biochemistry, Nagoya Women's University

• KIMURA Genjiro

  Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences

抄録

The objective of the present study was to establish whether high-density lipo-protein 3 (HDL₃) or high-density lipoprotein 2 (HDL₂) might show an anti-oxidative effect on the acceleration of the oxidative modification of low-density lipoprotein(LDL) by ascorbic acid from measurement of the agarose gel electrophoretic mobility of LDL. LDL was incu-bated without adding transitional-metal ions for 48 or 96h in phosphate-buffered saline (PBS) alone, with ascorbic acid (20μg/mL), or with both ascorbic acid (20μg/mL) and HDL₃ (200μg protein/mL). The LDL autoxidation occurred in PBS alone. Although ascor-bic acid significantly suppressed oxidative modification of LDL after incubation for 48h, the opposite was true after 96h. However, since the anti-oxidative ability of HDL₂ shows a weaker tendency than that of HDL₃, both HDL₃ and HDL₂ significantly inhibited this accel-eration of oxidative modification of LDL by ascorbic acid as assessed by electrophoretic mobility. If there is an augmented oxidative modification of LDL due to ascorbic acid in vivo, HDL₃ or HDL₂ may thus have an important role in inhibiting this ascorbic acid-accelerated oxidation of LDL.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ 51 (2), 75-79, 2005

  一般財団法人 学会誌刊行センター

キーワード

• oxidized low-density lipoprotein
• high density lipoprotein 3
• high density lipo-protein 2
• electrophoretic mobility
• ascorbic acid

詳細情報

• CRID: 1390282681302220672
• NII論文ID: 110002704034
• NII書誌ID: AA00703822
• DOI: 10.3177/jnsv.51.75
• ISSN: 18817742
• ISSN: 03014800
• ISSN: http://id.crossref.org/issn/03014800
• NDL書誌ID: 7311776
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7311776
• Web Site: https://search.jamas.or.jp/link/ui/2006262164
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可