Effects of Fasting and Refeeding on Expression of Proteolytic-Related Genes in Skeletal Muscle of Chicks
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- NAKASHIMA Kazuki
- Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science
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- KOMATSU Tokushi
- Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science
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- YAMAZAKI Makoto
- Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science
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- ABE Hiroyuki
- Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science
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This experiment was conducted to study the effects of fasting and refeeding on proteolytic-related gene expression in skeletal muscles of chicks. Chicks were fasted for 24h, and refed for h. Plasma Nτ-methylhistidine concentration, as an index of myofibrillar protein degradation, was increased by fasting, and that increment was reduced by refeeding. We also examined the expression of the protease mRNAs (calpain, proteasome, cathepsin and caspase-3) by real-time PCR of cDNA in skeletal muscles of fasting and refeeding chicks. Calpain (m-, μ-, and p94/calpain-3) mRNA expressions were also increased by fasting, and their increment was reduced by refeeding. Ubiquitin and 20S proteasome α subunit (α6 and α7) mRNA expressions as well as cathepsin B, and caspase-3 mRNA expression were likewise increased by fasting, with their increment also reduced by refeeding. These results indicate that fasting stimulates proteolytic-related gene expression, resulting in an increase in myofibrillar protein degradation, and that refeeding suppresses proteolytic-related gene expression, resulting in a decrease in myofibrillar protein degradation in chicks.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 51 (4), 248-253, 2005
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詳細情報 詳細情報について
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- CRID
- 1390001206323874688
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- NII論文ID
- 110002704071
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BD2MXhtVSktLzE
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- ISSN
- 18817742
- 03014800
- http://id.crossref.org/issn/03014800
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- NDL書誌ID
- 7412993
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可