ニホンナシから検出された種子および花粉伝染性の2本鎖RNA
書誌事項
- タイトル別名
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- Seed- and Pollen-transmitted Double-stranded RNAs Detected from Japanese Pear.
- Seed and Pollen-transmitted Double-stra
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Three nucleic acids were found in 5% polyacrylamide gel electrophoresed with extracts from several cultivars of Japanese pear (Pyrus spp.) that had no symptoms. They were cultivar-specific, and were detected from bark, leaf, petals and flowers plus petals. They were not digested by DNase I and were resistant to RNase A in 2×SSC buffer, but were susceptible to RNase A in 0.1×SSC buffer. These nucleic acids were absorbed by CF-11 cellulose in 15% ethanol. These results indicate that the three nucleic acids were double-stranded (ds) RNA. Molecular weights were estimated at about 1.08, 0.97, 0.90×106 daltons according to electrophoresis with dsRNAs of the rice dwarf virus as a marker. Several cultivars that have these dsRNAs had genetic relationships. The results of investigating the transmission of dsRNAs to the next generation by crossing showed that dsRNAs were detected in 31/40 when only the seed parent had dsRNAs, in 12/12 when only the pollen parent had dsRNAs, and in 5/5 when both parents had dsRNAs. These dsRNAs were transmitted high frequently to the next generation through both ovule and pollen, but transmission of dsRNAs by grafting to other cultivars without dsRNAs did not occur. These results suggest that the dsRNAs from Japanese pear are similar to dsRNAs derived from the phytocryptovirus group.
収録刊行物
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- 日本植物病理学会報
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日本植物病理学会報 64 (2), 110-115, 1998
日本植物病理学会
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詳細情報 詳細情報について
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- CRID
- 1390001206402877952
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- NII論文ID
- 110002732362
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- NII書誌ID
- AN0019269X
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- COI
- 1:CAS:528:DyaK1cXlvFSiu70%3D
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- ISSN
- 18820484
- 00319473
- http://id.crossref.org/issn/00319473
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- NDL書誌ID
- 4482250
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可