自然感染した野生植物から分離されたtobacco leaf curl geminivirusのAC1 ORF領域における遺伝子型変異
書誌事項
- タイトル別名
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- Genotypic Variability in AC1 ORF Region of Tobacco Leaf Curl Geminivirus from Naturally Infected Wild Plants.
- Genotypic Variability in AC1 ORF Region
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The wild plant Lonicera japonica, commonly found associated with tomato crops in Japan has been found to be infected with tobacco leaf curl virus (TLCV). Several novel species of virus-specific single-and double-stranded DNA (ss and dsDNA) were detected from infected plants showing yellow vein mosaic symptoms. Mung bean nuclease studies revealed the presence of unit-genome length duplexes of closed circular, relaxed circular and linear forms of viral DNA. In addition, a subgenomic DNA was also detected. Geminivirus-specific degenerate primers which anneal within the AC1 ORF (replicase gene) and the AV1 ORF (coat protein gene) amplified a fragment of 1.1 kbp, which was cloned and sequenced. Alignment of this sequence with that of TLCV showed complete homology in the intercistronic region, replicase and coat protein genes, indicating the presence of the same TLCV strain as detected from infected tomato. The geminivirus isolated from the common perennial Eupatorium makinoi from Saga Prefecture, Japan, showed 89.2% sequence homology in the AC1 ORF region with the TLCV strain detected in L. japonica. Infected wild plants therefore possess at least two distinct strains of TLCV which may serve as a natural reservoir for infecting crop species.
収録刊行物
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- 日本植物病理学会報
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日本植物病理学会報 63 (4), 298-303, 1997
日本植物病理学会
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詳細情報 詳細情報について
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- CRID
- 1390001206401867648
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- NII論文ID
- 110002734474
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- NII書誌ID
- AN0019269X
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- ISSN
- 18820484
- 00319473
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- NDL書誌ID
- 4305159
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
