リンゴステムグルービングウイルスLi-23分離株の全塩基配列とカリフラワーモザイクウイルス35S RNAプロモーターを含む感染性cDNAクローンの構築
書誌事項
- タイトル別名
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- Construction of an Infectious cDNA Clone of the Apple Stem Grooving Capillovirus (Isolate Li-23) Genome Containing a Cauliflower Mosaic Virus 35S RNA Promoter.
- Construction of an Infectious c DNA Clo
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The complete nucleotide sequence of the genome of apple stem grooving capillovirus (ASGV) isolate Li-23 from lily was determined. The isolate Li-23 genome consists of 6496 nucleotides excluding poly(A) tail and contains two overlapping open reading frames (ORFs 1 and 2). ORF1 encodes a protein with a Mr 241, 768 (242K) and ORF2, in a different frame within ORF1, encodes a Mr 36, 179 (36K) protein. The number of nucleotides and the arrangement of ORFs of the isolate Li-23 genome are in perfect agreement with those of isolate P-209 from apple and isolate L from lily. Identities of the nucleotide sequences were 98.4% (Li-23/L) and 83.0% (Li-23/P-209) in whole genomes excluding poly(A) tail. A full-length cDNA clone (pCT35SF) of the isolate Li-23 genome was cloned into a plasmid vector containing cauliflower mosaic virus 35S RNA promoter which was designed to transcribe the isolate Li-23 genome from its first nucleotide. Mechanical inoculation of pCT35SF onto Chenopodium quinoa induced symptoms typical of isolate Li-23 infection. Virus particles, viral RNA and coat protein could be detected in pCT35SF-inoculated plants, demonstrating that pCT35SF is infectious.
収録刊行物
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- 日本植物病理学会報
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日本植物病理学会報 63 (6), 432-436, 1997
日本植物病理学会
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詳細情報 詳細情報について
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- CRID
- 1390001206402473728
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- NII論文ID
- 110002734498
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- NII書誌ID
- AN0019269X
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- ISSN
- 18820484
- 00319473
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- NDL書誌ID
- 4393317
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
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- 抄録ライセンスフラグ
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