Measurement of K Vitamins in Food by High-Performance Liquid Chromatography with Fluorometric Detection

• SAKANO Toshiyuki

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• NOTSUMOTO Shigeru

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• NAGAOKA Tadayoshi

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• MORIMOTO Atsushi

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• FUJIMOTO Kyoko

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• MASUDA Sachiko

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• SUZUKI Yukiko

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

• HIRAUCHI Kazumasa

  Shionogi Research Laboratories, Shionogi & Co., Ltd.

Bibliographic Information

Other Title

• 蛍光検出高速液体クロマトグラフィー法による食品中のビタミンK類の測定
• ケイコウ ケンシュツ コウソク エキタイ クロマトグラフィーホウ ニ ヨル シ

Abstract

Vitamin K_1 (K_1) and vitamin K_2 (MK-n) in foods were measured by a method based on high-performance liquid chromatography (HPLC) combined with chemical reduction and fluorometric detection. The vitamins extracted from foods were purified with a silica SEP-PAK cartridge and/or thin-layer chromatography (TLC) and a silica SEP-PAK cartridge followed by TLC, respectively, and then measured by using an HPLC system. More than 1μg/g or ml of K_1 was found in salad oil, spinach leaf, green welsh onion, broccoli, purple laver, hijiki, wakame, green tea and black tea, and MK-n was mainly found in fermented foods.

Journal

• VITAMINS

  VITAMINS 62 (8), 393-398, 1988

  THE VITAMIN SOCIETY OF JAPAN

Keywords

• vitamin K_1
• vitamin K_2
• food
• HPLC
• fluorometric detection

Details

• CRID: 1390001205833851264
• NII Article ID: 110002847699
• NII Book ID: AN00207833
• DOI: 10.20632/vso.62.8_393
• ISSN: 2424080X
• ISSN: 0006386X
• NDL BIB ID: 3199237
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3199237
• Web Site: https://search.jamas.or.jp/link/ui/1989012903
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed