書誌事項
- タイトル別名
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- Determination of glucuronic acid in serum by HPLC.
- コウソク エキタイ クロマトグラフィー ニヨル ケッセイチュウ ノ グルクロン
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An analytical method for the determination of glucuronic acid in human serum was developed by high-performance liquid chromatography (HPLC) using an ion-pair method, and the optimum analytical conditions were investigated. This method involves the acidic degradation of 200 μl of human serum with 4 M sulfuric acid, neutralization with sodium hydroxide, a reaction with 0.5 M 3-metyl-1-phenyl-5-pyrazolone (PMP) methanol, and neutralization with 0.4 M sulfuric acid. Excess reaction regent was extracted with chloroform, and an aliquot of the aqueous solution was injected to the HPLC. By using an ion-pair reagent, glucuronic acid and glucose in human serum could be separated. Under the optimized analytical conditions, the calibration curve was linear over the range 550 mg/l. The recoveries of glucuronic acid from human serum samples at a level of 550 mg/l were 8294%. The intra-day and inter-day precision of the method were 0.64.3% (n = 5) and 4.36.9% (n = 5), respectively. The concentration of glucuronic acid in human serum was constant for 30 days at -20°C. This method was applied to the determination of glucuronic acid in human serum after single oral administration of glucuronolactone to volunteers.
収録刊行物
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- 分析化学
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分析化学 47 (2), 119-125, 1998
公益社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679029436800
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- NII論文ID
- 110002905582
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- NII書誌ID
- AN00222633
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- NDL書誌ID
- 4402698
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- ISSN
- 05251931
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可