Estrogenic activity of heavy oil and its assay method.

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Other Title
  • 重油のエストロゲン作用とその評価法
  • 重油のエストロゲン作用とその評価法 (<特集>環境ホルモンと分析化学)
  • ジュウユ ノ エストロゲン サヨウ ト ソノ ヒョウカホウ

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Abstract

The authors have proposed a new in vitro assay method for estrogenicity. The cellular progesterone receptor (PgR) level in the human breast cancer cell line MCF-7 was measured because PgR has been known to be upregulated by 17β-estradiol (E2). The cellular PgR level was measured by a western blotting technique. While an E2 treatment increased the PgR level, tamoxyfen, a typical E2 antagonist decreased the PgR level elevated by the E2 treatment. These results revealed that the cellular PgR level is a good indication to evaluate the estrogenicity. Next, the estrogenic activities of Nakhodka heavy oil and a commercial C-heavy oil were examined. Heavy oil crude extracts prepared with ethanol were subjected to the assay. While the extracts increased the PgR level under the E2-free condition, the extracts decreased the PgR level elevated by the E2 treatment. These results indicate that the heavy oil contains compounds which show weak estrogenic activity and act as E2 antagonists. Dead cells were observed after the oil extract treatment, and their number increased with an increase of oil extract concentration in the cell-culture medium. Environmental samples such as oil-extract, river water, and air particulate extract comprise a wide variety of compounds, and are thought to have a cell-killing effect. A cellular PgR expression method is considered to be more appropriate for assessing the estrogenic activities of environmental samples, compared with the existing assay methods, such as the cell-proliferation method (E-Screen assay) and the reporter-gene methods.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 48 (6), 617-622, 1999

    The Japan Society for Analytical Chemistry

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