ピロカテコールバイオレット-スズ(IV)錯体を用いるタンパク質の吸光光度定量 (<特集> バイオサイエンスと分析化学)

• 藤田 芳一

  大阪薬科大学

• 森 逸男

  大阪薬科大学

• 松尾 敬子

  大阪薬科大学

書誌事項

タイトル別名

• Biological Sciences and Analytical Chemistry. Spectrophotometric determination of protein with pyrocatechol violet-tin(IV) complex.
• バイオサイエンスと分析化学　ピロカテコールバイオレット‐スズ（ＩＶ）錯体を用いるタンパク質の吸光光度定量
• ピロカテコール バイオレット スズ 4 サクタイ オ モチイル タンパクシツ

抄録

A spectrophotometric method was established for the determination of protein with pyrocatechol violet-tin(IV) {PV-Sn(IV)} complex. This method can be used to determine up to about 200 μg/10 ml of human serum albumin (HSA), and is about 2 times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method). Results obtained for urinary protein by the PR method agree well with this method, the regression equation being Y (this method) =0.954X (PR method) +4.318 (n =35, r =0.986). A sensitive preconcentration method for spectrophotometry of trace protein which involves the filtration of an association complex [HSA-PV-Sn(IV)] on a membrane filter followed by its redissolution is described. The binding parameters (n and K) were calculated from a dual double-reciprocal plot. The thermodynamic parameters (ΔG, ΔH and ΔS) were obtained from a van't Hoff plot and the binding between HSA and PV-Sn(IV) complex was presumed to be a hydrophobic interaction.

収録刊行物

• 分析化学

  分析化学 44 (10), 733-738, 1995

  公益社団法人 日本分析化学会

キーワード

• protein determination by spectrophotometry
• pyrocatechol violet-tin(IV) complex
• preconcentation using a membrane filter
• thermodynamic parameter
• hydrophobic interaction

詳細情報

• CRID: 1390282679027783424
• NII論文ID: 110002906847
• NII書誌ID: AN00222633
• DOI: 10.2116/bunsekikagaku.44.733
• COI: 1:CAS:528:DyaK2MXovValsLc%3D
• NDL書誌ID: 3640176
• ISSN: 05251931
• ISSN: http://id.crossref.org/issn/05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3640176
• Web Site: http://www.jstage.jst.go.jp/article/bunsekikagaku1952/44/10/44_10_733/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10893676
• 本文言語コード: ja
• データソース種別:
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