Determination of cobalt in biological samples by trioctylamine extraction-atomic absorption spectrometry using a carbon tube atomizer

OYAMADA Noritaka, ISHIZAKI Mutsuo

doi:10.2116/bunsekikagaku.28.5_289

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Other Title

トリオクチルアミン抽出‐炭素管アトマイザ―原子吸光法による生体試料中のコバルトの定量
トリオクチルアミンチュウシュツタンソカンアトマイザーゲンシキュウコ
トリオクチルアミン抽出-炭素管アトマイザー原子吸光法による生体試料中のコバルトの定量

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Abstract

Millimicrogram amounts of cobalt in biological samples were determined by atomic absorption spectrometry using carbon tube atomizer after the pretreatment described as follows. The proposed procedure is as follows : (15)g of viscera or flesh was taken into a porcelain crucible, which had been heated in advance in a muffle furnace for 2 h at 550°C, and it was dried for 12 h at 60°C. In the case of urine, 5 ml of the sample was taken into a collodion membrane tube containing 150 mg of sephadex G-200, and it was dried in a drying oven for 12 h at 60°C suspending by thread. The dried sample was ashed by heating at 550°C for 6h. The ashed samples were dissolved in 10 ml of 10 N hydrochloric acid, and it was transferred to a separatory funnel. Cobalt was extracted with 10 ml of 5% carbon tetrachloride solution of trioctylamine, and then it was back extracted with 1 ml of distilled water. The back extract was injected into the carbon tube and the cobalt content in it was determined by atomic absorption spectrometry. By this process, cobalt in biological samples could be determined with a recovery of (98103)%, and with the variation coefficient of less than 11%.

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 28 (5), 289-293, 1979

The Japan Society for Analytical Chemistry

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