ポリ（エチレンイミン）被覆シリカゲルミクロボアカラムによるフェノール及び芳香族有機酸のクロマトグラフィー

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書誌事項

タイトル別名
  • Microbore packed column liquid chromatography of phenols and aromatic acids on poly (ethyleneimine) coated silica gel.
  • ポリ エチレンイミン ヒフク シリカ ゲル ミクロボア カラム ニ ヨル フェ

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抄録

Poly(ethyleneimine) (PEI 6) was coated on porous silica gel beads in a methanol solution and packed into a stainless steel tube (140220 mm × 0.8 mm i.d.) by a slurry packing method. The surface coated PEI 6 was cross-linked with ethyleneglycole diglycidylether. A Shimadzu Model LC 5A liquid chromatograph with a SPD-2AM UV detector was used. PEI 6 was coated on a Develosil 5 (5 μm, Nomura Chemicals, Japan) or LiChrospher SI 100 (10 μm, E. Merck, F. R. G.). Phenolic, benzoic, and benzenesulfonic compounds were chromatographed with 2.515 mM carbonate or phosphate eluents. This column showed an excellent separation of o-, m-, and p-isomers of above compounds. The capacity factors, k', and chromatograms of over 30 compounds eluted from the column are reported. The k' values of dinitrophenols increased with the eluent pH at low pH range and had maximum values between pH 4 and 5, above this pH range, the values decreased with increasing pH of the eluent. This may be explained by the complex effects of the dissociation of solute molecules, counter ions in the eluent and secondary or tertiary ammonium residues in the stationary phase. By cross-linking of the coated PEI 6 on the silica gel, retention of nitrophenols was increased and elution order or isomers was altered. The cross-linked PEI 6 coated silica gel column will be useful for the separation of isomeric phenols and aromatic acids. This column was stable and good reproducibility was obtained after continuous operation of about three months.

収録刊行物

  • 分析化学

    分析化学 33 (9), 481-485, 1984

    公益社団法人 日本分析化学会

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