高速液体クロマトグラフィーによる塩酸アヘンアルカロイドの定量(<特集>医薬品の分析)

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書誌事項

タイトル別名
  • Determination of opium alkaloid hydrochloride by high performance liquid chromatography.
  • 高速液体クロマトグラフィーによる塩酸アヘンアルカロイドの定量
  • コウソク エキタイ クロマトグラフィー ニ ヨル エンサン アヘン アルカロイ

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抄録

A rapid and sensitive high performance liquid chromatographic method was established for simultaneous determination of the five main alkaloids (morphine, codeine, narceine, noscapine, papaverine) in opium alkaloid hydrochloride (JPX). A sample was separated on a column (150 mm × 4.6 mm i. d.) packed with reversed phase (Hitachi Gel # 3056), and eluted with a mixture of acetonitrile, 0.005 M phosphate buffer (pH 3) and 0.2 M heptanesulfonate (50 : 50 : 1.5) as the mobile phase. The effluent was monitored with a ultraviolet detector at a wavelength of 254 nm. Determination was carrid out successfully by using ethylmorphine hydrochloride as an internal standard. Recoveries of these alkaloids for known standard sample were satisfactory. The relative standard deviation for the determination of these alkaloids was less than 1.5%. This chromatographic method is simple, accurate and selective with an analysis time of less than 15 min.

収録刊行物

  • 分析化学

    分析化学 35 (3), 173-176, 1986

    公益社団法人 日本分析化学会

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