Determination of (trans-l-1,2-diaminocyclohexane) oxalatoplatinum(II) in plasmaand urine by high performance liquid chromatography.

• KIZU Ryoichi

  Faculty of Pharmaceutical Sciences, Kanazawa University

• HIGASHI Shin-ichi

  Faculty of Pharmaceutical Sciences, Kanazawa University

• HAYAKAWA Kazuichi

  Faculty of Pharmaceutical Sciences, Kanazawa University

• MIYAZAKI Motoichi

  Faculty of Pharmaceutical Sciences, Kanazawa University

Bibliographic Information

Other Title

• 高速液体クロマトグラフィーによる血しょう及び尿中のオキザラト（トランス‐ｌ‐１，２‐ジアミノシクロヘキサン）プラチニウム（ＩＩ）の定量
• コウソク エキタイ クロマトグラフィー ニ ヨル ケッショウ オヨビ ニョウチ
• 高速液体クロマトグラフィーによる血しょう及び尿中のオキザラート(トランス-l-1,2-ジアミノシクロヘキサン)プラチニウム(II)の定量(<特集>医薬品の分析)

Abstract

A method for determination of (trans-l-1, 2-diaminocyclohexane) oxalatoplatinum (II) (l-OHP), introduced by Kidani et al. as one of the promising antineoplastic platinum complexes, in plasma and urine by high performance liquid chromatography (HPLC) was investigated in order to contribute for metabolic study of l-OHP. The recommended pretreatment procedures and HPLC conditions are as follows. Pretreatment of plasma and urine ; pretreatment column is composed of Dowex 1-X8 (5 mm i.d. × 6 cm), Dowex 50 W-X4 (5 mm i.d. x 3 cm) and Sep-Pak C₁₈ cartridge. Blood samples are centrifuged at 1000 g for 3 min to obtain plasma. One ml of plasma or urine is immediately placed on the pretreatment column and is eluted with water at the flow rate of 1.2 ml/min. The initial 6 ml portion of effluent is discarded and subsequent 10 ml portion is collected. An aliquot (100 μl) of the effluent collected is subjected to HPLC. All of these pretreatment procedures are carried out at 5±2°C. HPLC conditions ; column : Finepak SIL C₁₈ (4.6 mm i.d. × 25 cm), eluent : H₂O-MeOH (95 : 5), column temperature : 40°C, flow rate : 1 ml/min, detection wavelength : 210 nm, injection volume : 100μl. Under the conditions described above, a linear relationship was obtained between peak height and l-OHP concentration in plasma and urine. Determination limit was 0.5 μM l-OHP in sample solutions. Recovery of l-OHP in pretreatment was 86±3% in plasma and 83±2% in urine. A sensitive and precise determination of l-OHP in plasma and urine will be possible by this method.

Journal

• BUNSEKI KAGAKU

  BUNSEKI KAGAKU 35 (3), 250-255, 1986

  The Japan Society for Analytical Chemistry

Keywords

• (trans-l-1,2-diaminocyclohexane) oxalatoplatinum (II)
• antineoplastic platinum complex
• high performance liquid chromatographic determination
• plasma and urine

Details

• CRID: 1390282679029828992
• NII Article ID: 110002909323
• NII Book ID: AN00222633
• DOI: 10.2116/bunsekikagaku.35.3_250
• NDL BIB ID: 3081020
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3081020
• Web Site: http://www.jstage.jst.go.jp/article/bunsekikagaku1952/35/3/35_3_250/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10891180
• Web Site: https://search.jamas.or.jp/link/ui/1987012930
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
• Abstract License Flag: Disallowed