全血液及び血清中グルタチオンのアンペロメトリー

• 木下 英明

  活水女子大学人間関係学科

書誌事項

タイトル別名

• Amperometric Assay of Glutathione in Whole Blood and Serum
• ゼン ケツエキ オヨビ ケッセイ チュウ グルタチオン ノ アンペロメトリー

抄録

The anodic steady-state current by a sulfhydryl compound in concentrations about 10 μM at a dialysis membrane covered phthalocyanine-embedded carbon paste electrode (mCoPCPE) completely vanished after the addition of N -ethylmaleimide (NEM) in double concentrations of the sulfhydryl compounds. The concentrations of reduced glutathione (GSH) in whole blood and serum were determined by measuring the current decrease at 150 mV after the addition of NEM in blood solutions of pH 6.8. This method has the advantage of eliminating the procedures for deproteinization. The correlation coefficients between the GSH concentration determined in this method and those by a spectrometric measurement were 0.98 and 0.90 for 10 whole blood and 15 serum samples, respectively<br>

収録刊行物

• 分析化学

  分析化学 54 (1), 71-74, 2005

  公益社団法人 日本分析化学会

キーワード

• glutathione
• amperometry
• membrane-covered electrode
• whole blood
• serum

詳細情報

• CRID: 1390001204356471168
• NII論文ID: 110002910023
• NII書誌ID: AN00222633
• DOI: 10.2116/bunsekikagaku.54.71
• COI: 1:CAS:528:DC%2BD2MXmsVSnug%3D%3D
• NDL書誌ID: 7217208
• ISSN: 05251931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7217208
• Web Site: http://www.jstage.jst.go.jp/article/bunsekikagaku/54/1/54_1_71/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10896014
• Web Site: https://search.jamas.or.jp/link/ui/2005084057
• 本文言語コード: ja
• データソース種別:
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