Characterization and Environmental Regulation of a 24 kDa Cornus sericea Dehydrin-like Protein and its Relationship to Freeze-Tolerance

• KARLSON Dale

  Department of Horticulture and Landscape Architecture, Purdue University:(Present address)Division of Plant and Soil Sciences, West Virginia University

• SARNIGHAUSEN Eric

  Department of Horticulture and Landscape Architecture, Purdue University:(Present address)Institut fur Biologie II, Albert-Ludwigs Universitdt Freiburg

• ZENG Yan

  Department of Horticulture and Landscape Architecture, Purdue University:(Present address)VA Medical Center

• ITOH Takao

  Wood Research Institute, Kyoto University

• BABA Kei'ichi

  Wood Research Institute, Kyoto University

• FUJINO Takeshi

  Wood Research Institute, Kyoto University

• JOLY Robert

  Department of Horticulture and Landscape Architecture, Purdue University

• RHAGOTHAMA K. G.

  Department of Horticulture and Landscape Architecture, Purdue University

• STIRM Vicki

  Department of Horticulture and Landscape Architecture, Purdue University

• ASHWORTH Edward

  Department of Horticulture and Landscape Architecture, Purdue University

書誌事項

タイトル別名

• Characterization and Environmental Regulation of a 24 kDa Cornus sericea Dehydrin-like Protein and its Relationship to Freeze-Tolerance (The Seminar, "Stresses and Responses and Adaptation of Living Organisms : Molecular and Cellular Mechanisms and its Application")

抄録

Using the extremely freeze-tolerant woody shrub C. sericea as a model system, we investigated the association of xylem proteins to cold acclimation. We identified a predominant 24 kDa dehydrin-like protein that accumulated during cold acclimation, raised a polyclonal antibody and screened a cDNA expression library to search for related genes. In controlled environment conditions, we identified and deciphered the mode of regulation for the 24 kDa protein by manipulating abiotic factors and monitoring subsequent physiological and molecular effects. With immunolocalization techniques, we clarified the subcellular localization of 24 kDa-like proteins in an effort to provide insight into a potential biological role. To assess the proposed role of dehydrins to freeze induced desiccation (caused by extracellular ice formation), we characterized freezing behavior within 30 Cornus species into classifications of either supercooling or non-supercooling behaviors. Subsequent comparisons of total xylem protein blot analysis determined that 24 kDa-like proteins were not limited to species that display non-supercooling behavior, nor to those species that are extremely freeze-tolerant.

収録刊行物

• 低温生物工学会誌

  低温生物工学会誌 49 (1), 29-35, 2003

  低温生物工学会

キーワード

• Cornus sericea
• デハイドリン
• 耐凍性
• 免疫局在性
• 光周性

詳細情報

• CRID: 1390001205087756544
• NII論文ID: 110002913976
• NII書誌ID: AN10448734
• DOI: 10.20585/cryobolcryotechnol.49.1_29
• ISSN: 24241555
• ISSN: 13407902
• NDL書誌ID: 6730525
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6730525
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可