口腔癌細胞核 DNA 量に関する研究 : 術前治療による核 DNA 量の変動

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  • A Study of Nuclear DNA Content of Oral Carcinoma Cell : Change of Nuclear DNA Content by Pre-operation Treatment

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Nuclear DNA contents of oral squamous cell carcinoma cells were measured using MMSP-TU system (Microphotometry method). At first, the methods of material treatments were discussed and in 19 cases who were all had oral squamous cell carcinoma, the changes of nuclear DNA content after neo-adjuvant chemotherapy and radiation therapy were studied. The results were as follows. 1. When thin sections of 5μm, 10μm, and of cell isolation of 30μm were compared, no significant differences were found in the mean values of nuclear DNA content and a 90% agreement was shown between 10μm and cell isolation. Sections of 10μm therefore were used for this study. 2. In changes in nuclear DNA pattern by pre-operation treatment, 6 cases showed a shift toward left, 11 cases no change and 2 cases a shift toward right. In 6 cases whose nuclear DNA pattern shifted toward left, the means of nuclear DNA content of 5 cases were decreased (those of 3 cases were significantly decreased). In 2 cases whose nuclear DNA pattern shifted toward right, the means of nuclear DNA contents of all cases were increased. 3. In the group which used 5-FU at pre-operation, 9 of 12 cases showed decrease of the means of nuclear DNA content (those of 4 cases were significantly decreased). On the other hand, in the group which did not use 5-FU at pre-operation, only 3 of 7 cases showed decrease (there were not significantly decreased). 4. On the histological effect by pre-operation treatment, 4 of 6 cases whose nuclear DNA pattern shifted toward left showed over-GIII. 9 of 10 cases whose histological effect was over-GIIb (effective) showed decrease of the means of nuclear DNA content. In contrast, 6 of 9 cases whose histological effects were G I and G IIa (non-effective) showed increase of the means of nuclear DNA content. 5. By pre-operation treatment, 71.4% of the cases whose nuclear DNA pattern was type IV at biopsy showed effective (over PR). This was higher as compared to 63.6% of type III. But in the cases of type IV, there were histologically more invasive cases and so the prognosis was worse. Especially, in 2 cases were type IV of nuclear DNA pattern at biopsy and showed increase of the means of nuclear DNA content by pre-operation treatment, prognosis was not good. 6. In 7 of 8 cases whose gross pattern was ulcer type, nuclear DNA pattern did not change by pre-operation treatment and 4 cases of these were histologically non-effective (MR or NC). 7. In 6 of 7 cases whose nuclear DNA pattern showed type IV, durations were within 2 months. In 6 cases whose gross pattern was ulcer type and whose durations were within 2 months, all showed T3 or T4 and 3 cases had metastases of regional lymph nodes. It is suggested that nuclear DNA content is one of the available parameters to estimate histological effect by pre-operation treatment and that nuclear DNA content is a high risk factor in prognosis.

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