Introduction and Expression of Glucose Transporters in Pancreatic Acinar Cells by In Vivo Electroporation

• Shinoda Yasuo

  Department of Anatomy and Cell Biology, Gunma University School of Medicine First Department of Surgery, Gunma University School of Medicine

• Matsuzaki Toshiyuki

  Department of Anatomy and Cell Biology, Gunma University School of Medicine

• Yokoo-Sugawara Minako

  Third Department of Internal Medicine, Gunma University School of Medicine

• Suzuki Takeshi

  Department of Anatomy and Cell Biology, Gunma University School of Medicine

• Aoki Takeo

  Department of Anatomy and Cell Biology, Gunma University School of Medicine

• Hagiwara Haruo

  Department of Anatomy and Cell Biology, Gunma University School of Medicine

• Kuwano Hiroyuki

  First Department of Surgery, Gunma University School of Medicine

• Takata Kuniaki

  Department of Anatomy and Cell Biology, Gunma University School of Medicine

抄録

cDNAs encoding facilitated-diffusion glucose transporters GULT1, GULT3, GULT4, and GULT5 were introduced into the rat pancreas by in-vivo electroporation method, and their expression and localization in pancreatic acinar cells were examined immunohistochemically. GLUT1 was localized at the basolateral membrane, whereas GLUT3 and GLUT5 were at the apical membrane. Restriction of GLUT3 and GLUT5 to the apical membrane makes a marked contrast to their localization to the entire plasma membrane when expressed in hepatocytes in situ. Such differential localization may be due to differential apical targeting mechanism: direct targeting in acinar cells and indirect transcytotic delivery in hepatocytes in situ. GLUT4 was present in the membrane of zymogen secretory granules in the cytoplasm in acinar cells. This observation suggests that GLUT4 is segregated to the regulated secretory pathway. Expression of glucose transporters in situ is a useful method in analyzing the targeting mechanism of membrane proteins in cells of tissues in situ.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 36 (1), 77-82, 2003

  日本組織細胞化学会

キーワード

• glucose transporters
• <i>in-vivo</i> electroporation
• pancreas

詳細情報

• CRID: 1390001204862412032
• NII論文ID: 110003144258
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.36.77
• COI: 1:CAS:528:DC%2BD3sXjvVSgsbo%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/36/1/36_1_77/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10804392
• Web Site: https://search.jamas.or.jp/link/ui/2003272682
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可