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抄録
シシャモ集団の遺伝的特性把握を目的に4河川集団, 91個体のmtDNAについて9種の制限酵素によるRFLP解析を行い, 17のハプロタイプを検出した。ハプロタイプ間および集団間の塩基置換率はそれぞれ0.27∿2.33%, 0.37∿0.54%。形態形質から唱えられていた系群間において本研究でハプロタイプ頻度に有意差が観察された。
Mitochondrial DNA (mt DNA) of Japanese Longfin Smelt Spirinchus lanceolatus among four river populations were examined to clarify their genetic feature and population structure. Restriction fragment length polymorphism (RFLP) analysis using 9 kinds of 6-base recognized restriction endonucleases (Apa I, Ava I, BamH I, Bgl II, BstE II, EcoR I, Pst I, Pvu II, Xba I) revealed considerable variaton. We found 17 haplotypes in 91 specimens. Restriction fragment patterns were polymorphic for 7 enzymes examined. Nucleotide divergence between haplotypes ranged from 0.27 to 2.33%. Nucleotide divergence between populations ranged from 0.37 to 0.54%. A dendrogram constructed by UPGMA among 17 haplotypes showed no clustering groups of localities. Nucleotide diversity and nucleon diversity of each locality ranged from 0.0028 to 0.0052,and from 0.7758 to 0.9074,respectively. Distinguishable two stocks by morphological analysis showed significant difference in their haplotype freqencies.
