Limitted Degradation of .ALPHA.-actinin in the Ordinary Muscle of Cultured Red Sea Bream during Storage in Ice.
Bibliographic Information
- Other Title
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- 養殖マダイの氷蔵中における普通筋中α‐アクチニンの限定分解
- 養殖マダイの氷蔵中における普通筋中α-アクチニンの限定分解
- ヨウショク マダイ ノ ヒョウゾウ チュウ ニ オケル フツウキン チュウ アルファ アクチニン ノ ゲンテイ ブンカイ
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Abstract
Anti-α-actinin IgG was prepared from rat serum immunized with purified α-actinin in the ordinary muscle of red sea bream. Anti-α-actinin IgG showed immune reactivity against not only α-actinin in ordinary muscle but also papain-digests of the α-actinin. According to immunoelectron microscopic method, α-actinin was detected on Z line of ordinary muscle cells and non-specific reactions were not detected on another organelles. The changes of α-actinin in the ordinary muscle of cultured red sea bream during storage in ice were examined by SDS-PAGE followed by its immunoblotting. It was found that the limited digestion of α-actinin in ordinary muscle of cultured red sea bream started on day one in ice, then, native α-actinin was not detected on day 5 and 7. These results suggested that the weakening of Z-disks in ordinary muscle was caused by the disappearance of α-actinin and resulted in the softness of the flesh texture of cultured red sea bream.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 67 (4), 723-727, 2001
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390282681389109760
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- NII Article ID
- 110003145329
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- HANDLE
- 10069/21551
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- NDL BIB ID
- 5848825
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed