A procedure for making precision stereotaxic surgery in the goldfish

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  • procedure for making precision stereotaxic surgery in the goldfish

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A new device and a surgical procedure for placing electrodes in goldfish are described. They are used in stereotaxic surgery that is performed in lesion and electrophysiology studies. We modified DKI's stereotaxic apparatus in which the subject is fixed on a mouthpiece and two rubber plates in order to produce highly localized lesions in fish. A body holder and a new method of administering anesthesia were also developed. By stabilizing the water flow into the subject's gill using a three-pronged pump, we were able to maintain the anesthetic effect.

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