Platelet-type Arachidonate 12-Lipoxygenase in Mouse Gastrointestinal Tract.

• Nakamura Michihiro

  Department of Biochemistry, Tokushima University, School of Medicine

• Yamamoto Shozo

  Department of Biochemistry, Tokushima University, School of Medicine

• Ishimura Kazunori

  Department of Anatomy, Tokushima University, School of Medicine

抄録

12-Lipoxygenase enzyme oxygenates the position 12 of arachidonic acid, and produces 12S-hydroperoxy-arachidonic acid. When mouse gastrointestinal tract was immunostained with an antiserum against human platelet 12-lipoxygenase and examined by light microscopy, positively-stained cells were found in the epithelium of stomach and small and large intestines. Electron microscopic immunohistochemistry revealed that the positively-stained cells were open-type enteroendocrine cells. The endocrine cells had many granules of heterogeneous sizes, forms and internal structures, and most of the granules had electron lucent area. 12-Lipoxygenase was localized in the cytoplasm, but not in the nucleus, plasma membranes and other subcellular organelles. Double immunostaining revealed that all 12-lipoxygenasepositive cells contained serotonin. With an antiserum against 12-lipoxygenase isozyme of porcine leukocytes, no positively-stained cells were observed in gastrointestinal epithelium.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 31 (1), 71-75, 1998

  日本組織細胞化学会

キーワード

• 12-Lipoxygenase
• Arachidonic acid
• Serotonin
• Mouse intestine
• Immunohistochemistry

詳細情報

• CRID: 1390001204860977920
• NII論文ID: 110003146810
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.31.71
• COI: 1:CAS:528:DyaK1cXjtVWrt7w%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/31/1/31_1_71/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10803055
• Web Site: https://search.jamas.or.jp/link/ui/1998173154
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可