Ultrastructural Localization of Hair Keratins in Human Scalp Hair Shafts as Revealed by Rapid-Freezing Immunocytochemistry.
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- Takizawa Takami
- Department of Anatomy, Jichi Medical School Cosmetics Laboratory, Kanebo Ltd.
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- Takizawa Toshihiro
- Department of Anatomy, Jichi Medical School
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- Uchiwa Hideyo
- Basic Research Laboratory, Kanebo Ltd.
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- Arai Seiichi
- Cosmetics Laboratory, Kanebo Ltd.
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Although many hair proteins have been investigated biochemically, little information is available on their subcellular distribution in human hair. We report here on the immunoelectron microscopic technique for defining the ultrastructural localization of hair proteins, especially hair keratins, in human scalp hair shafts: a combination of a rapid-freezing, freeze-substitution fixation without chemical fixatives, and subsequent immunocytochemistry (i. e., rapid-freezing immunocytochemistry). The hair shafts were rapid-frozen, freeze-substituted in acetone without chemical fixatives, and then embedded in LR White resin. Subsequently, ultrathin-sectioned samples were stained for hair keratins by an immunogold technique. Rapid-freezing followed by freeze-substitution without chemical fixatives well-preserved not only the fine structure of the hair shafts but also the antigenicity of hair keratins for immunocytochemistry. In the cortex, hair keratins were present mainly on the macrofibrils. In the cuticle, they were also located primarily in the endocuticle, which did not show the fibrous structure like the macrofibrils did. Rapid-freezing immunocytochemistry appears to be the most viable approach for revealing the macromolecular architecture of human hair, which is a completely keratinized tissue and one of the most delicate tissues in preparation for transmission electron microscopy.
収録刊行物
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- Acta Histochemica et Cytochemica
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Acta Histochemica et Cytochemica 31 (3), 237-242, 1998
日本組織細胞化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679839970176
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- NII論文ID
- 110003146971
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- NII書誌ID
- AA00508022
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- COI
- 1:CAS:528:DyaK1cXlsVCnt70%3D
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- ISSN
- 13475800
- 00445991
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可