Cytochemical localization of Mg++-ATPase and Ca++-ATPase on the limiting membrane of rat liver peroxisomes.

• MAKITA TAKASHI

  Veterinary Anatomy, Yamaguchi University

• HAKOI KAZUO

  Veterinary Anatomy, Yamaguchi University

• ARAKI NOBUKAZU

  Department of Anatomy, Ehime University School of Medicine

抄録

By a modified cytochemical staining procedure, Mg⁺⁺- and Ca⁺⁺-ATPase were clearly localized on the surface of the limiting membrane of peroxisomes in hepatocytes of the rat. The matrix and core structure (crystalloid) were devoid of the ATPase reaction. The reaction on the peroxisomal membrane was abolished by an inhibitor of Mg⁺⁺-ATPase, 10mM p-chloromercuric benzoate (PCMB) and by the omission of the substrate, ATP-2Na, or by replacement of it with β-glycerophosphate. The reaction was not suppressed by the inhibitor of a alkaline phosphatase, 2.5mM levamisole; by an inhibitor of Na-K-ATPase, 10mM ouabain. After replacement of MgSO₄ by CaCl₂, Ca-ATPase was localized at exactly the same site where Mg-ATPase was detected.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 23 (5), 601-611, 1990

  日本組織細胞化学会

キーワード

• Cell Biology
• Histology
• Physiology
• Biochemistry
• Pathology and Forensic Medicine

詳細情報

• CRID: 1390001204861337344
• NII論文ID: 110003147555
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.23.601
• COI: 1:CAS:528:DyaK3MXhsVSgsr0%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/23/5/23_5_601/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10802752
• Web Site: https://search.jamas.or.jp/link/ui/1993232478
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可