STUDIES ON TRANSLOCATION OF IMMUNOGLOBULINS ACROSS INTESTINAL EPITHELIUM

DOI NDLデジタルコレクション Web Site 被引用文献36件
  • ISOBE YOSHINARI
    The Departments of Medicine (Gastroenterology) and Pathology of the Denver Veterans Administration Hospital University of Colorado Medical Center
  • CHEN SHIN-TSE
    The Departments of Medicine (Gastroenterology) and Pathology of the Denver Veterans Administration Hospital University of Colorado Medical Center
  • NAKANE PAUL K.
    The Departments of Medicine (Gastroenterology) and Pathology of the Denver Veterans Administration Hospital University of Colorado Medical Center
  • BROWN WILLIAM R.
    The Departments of Medicine (Gastroenterology) and Pathology of the Denver Veterans Administration Hospital University of Colorado Medical Center

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  • I. IMPROVEMENTS IN THE PEROXIDASE-LABELED ANTIBODY METHOD FOR APPLICATION TO STUDY OF HUMAN INTESTINAL MUCOSA

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We adapted the peroxidase-labeled antibody method to localization of immunoglobulins and the secretory component (SC) in human intestinal mucosa at the light and electron microscopic levels. Modifications of the method included (1) the use of digitonin in the first wash step of tissues fixed in periodate-lysine-paraformaldehyde; (2) double coating of glass slides with gelatin and egg albumin before application of cyostat sections, and (3) inactivation of endogenous peroxidase by periodic acid treatment of cyostat sections. With these improvements, tissue morphology was well preserved; antigenicity of immunoglobulins and SC was retained; penetration of labeled antibodies into cellular compartments was complete; and confusion at the light microscopic level between peroxidase-containing cells and cells stained with peroxidase-labeled antibodies was avoided.

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