Actin filaments of hepatocytes in experimental rat cholestasis - Observations using a fluorescent staining method by DACM labeled heavy meromyosin.

DOI NDLデジタルコレクション Web Site 被引用文献1件
  • TAMURA KOHEI
    Liver Unit, Department of Medicine, Juntendo University School of Medicine
  • KURODA HIROYUKI
    Liver Unit, Department of Medicine, Juntendo University School of Medicine
  • WATANABE SUMIO
    Liver Unit, Department of Medicine, Juntendo University School of Medicine
  • YOKOI YUKIO
    Liver Unit, Department of Medicine, Juntendo University School of Medicine

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Actin filaments of hepatocytes in three types of experimental rat cholestasis were examined by a previously reported method (J. Histochem. Cytochem. 28; 335, 1980). Liver treated with cytochalasin B in vivo showed bile canalicular dilation and the obscure polygonal pattern of hepatocytes depended upon partial loss and granular aggregation in peripheral fluorescence. Liver treated with phalloidin showed an accumulation of fluorescence mainly around bile canaliculi in addition to an accumulation in the cytoplasma and nucleus in hepatocytes. Liver ligated with common bile duct showed increased fluorescence in the peripheral cytoplasma of hepatocytes, particularly around bile canaliculi, but this alteration varied according to the duration of ligation.

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