Peroxidatic activity of catalase in the sea urchin ovary.

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Endogenous catalase activity in the ovary of two sea urchin species, Hemicentrotus pulcherrimus and Anthocidalis crassispina, was investigated by 3, 3′-diaminobenzidine (DAB) method.<br>Osmicated DAB products were located at the lamellar (in H. pulherrimus) or stellate (in A. crassispina) structure of the cortical granule (Cg) in the mature egg and that of the presumptive cortical granule (pCg) in the oocyte.<br>As for the A-type giant granule (A-g.) in the nurse cell, the same product was also found at the cortical granule-like structure (Cgls), but not at the other part of A-g.. In the present study of sea urchin ovary it is clear that the A-g. is “Heterophagosome”, and that the heterophagic substance may be regenerated as a nutrient for the growing oocyte after autolysis of nurse cell.

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