LOCALIZATION OF ANTI-ALLERGIC AGENT IN RAT MAST CELLS DEMONSTRATED BY LIGHT AND ELECTRON MICROSCOPIC RADIOAUTOGRAPHY

• NAGATA TETSUJI

  Department of Anatomy, Shinshu University School of Medicine Central Research Laboratories, Kissei Pharmaceutical Co., Ltd.

• NISHIGAKI TOSHIAKI

  Department of Anatomy, Shinshu University School of Medicine Central Research Laboratories, Kissei Pharmaceutical Co., Ltd.

• MOMOSE YASUNORI

  Department of Anatomy, Shinshu University School of Medicine Central Research Laboratories, Kissei Pharmaceutical Co., Ltd.

抄録

In order to demonstrate the intracellular localization of anti-allergic agent in rat mast cells, ³H-labeled Tranilast, N-(3', 4'-dimethoxycinnamoyl) anthranilic acid was studied by both light and electron microscopic radio-autography.<br>The peritoneal mast cells of several Wistar strain rats were collected and incubated in vitro with Tranilast (100μM) after sensitization with IgE and challenging with an antigen, demonstrating the inhibition of degranulation by type I allergic reaction. Then, the peritoneal mast cells were incubated in vitro with ³H-Tranilast (100μCi/ml) for varying time intervals from 0 to 60min and the cell pellets were fixed, embedded and processed for light and electron microscopic radioautography. Light microscopic radioautograms revealed that silver grains were localized over the cytoplasm. Electron microscopic radioautograms revealed that silver grains were localized over and around the granules. The number of silver grains increased according to the prolongation of incubation time.<br>Several rats were administered orally with ³H-Tranilast (53μCi/gm body weight). They were sacrificed at various time intervals and the back skins were taken out, fixed, embedded and processed for light and electron microscopic radioautography. Silver grains were observed to localize over the subcutaneous tissues, especially concentrating over the mast cells.<br>From the results, it is concluded that ³H-Tranilast was rapidly taken into mast cells in both in vitro and in vivo and it inhibited degranulation of type I allergic reaction.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 19 (5), 669-683, 1986

  日本組織細胞化学会

キーワード

• Cell Biology
• Histology
• Physiology
• Biochemistry
• Pathology and Forensic Medicine

詳細情報

• CRID: 1390282679840090880
• NII論文ID: 110003150019
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.19.669
• COI: 1:CAS:528:DyaL2sXhsVyqsb0%3D
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/19/5/19_5_669/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10801853
• Web Site: https://search.jamas.or.jp/link/ui/1988000848
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可