Insulin-Like Growth Factor-I and Its Receptor in Mouse Pituitary Glands

• Honda, Junichi

• Takeuchi, Sakae

• Fukumachi, Hiroshi

• Takahashi, Sumio

Bibliographic Information

Other Title

• Insulin-like growth factor-1 and its receptor in mouse pituitary glands
• Insulin-like growth factor-1 and its re
• Insulin-Like Growth Factor-l and Its Receptor in Mouse Pituitary Glands.

Abstract

Insulin-like growth factor-I (IGF-I) is produced in the liver and other peripheral tissues in response to growth hormone (GH) stimuli. IGF-I regulates diverse physiological functions in an autocrine and/or paracrine manner. IGF-I and IGF-I receptor (type-I receptor) are expressed in human and rat pituitary glands. However, the cell types of IGF-I-expressing cells and target cells of IGF-I in the pituitary glands are not known. The present study was aimed to identify the cell types of IGF-I-expressing cells and of its type-I receptor-expressing cells in mouse pituitary glands. In the mouse pituitary glands, IGF-I mRNA and IGF-I receptor mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). IGF-I-expressing cells and its receptor-expressing cells were detected by non-radioisotopic in situ hybridization using mouse IGF-I cDNA and IGF-I receptor cDNA probes, and their cell types were immunocytochemically determined using antibodies raised against pituitary hormones. We found that somatotrophs expressed both IGF-I and IGF-I receptors, and some of corticotrophs expressed IGF-I receptors. Co-localization of IGF-I and GH in the same cultured pituitary cells was observed by dual-labelling immunocytochemistry. The present study demonstrated that pituitary IGF-I produced in somatotrophs regulated functions of somatotrophs and corticotrophs in an autocrine and/or paracrine manner.

Journal

• Zoological Science

  Zoological Science 15 (4), 573-579, 1998-08

  Zoological Society of Japan

Keywords

• Animal Science and Zoology

Details

• CRID: 1050283687312453376
• NII Article ID: 110003323592
• NII Book ID: AA10545874
• ISSN: 02890003
• DOI: 10.2108/zsj.15.573
• NDL BIB ID: 4555875
• Web Site: https://ousar.lib.okayama-u.ac.jp/47702
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4555875
• Web Site: http://www.bioone.org/doi/pdf/10.2108/zsj.15.573
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10861518
• Web Site: https://search.jamas.or.jp/link/ui/1999022952
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles