A Cell Line Derived from the Fin of the Goldfish, Carassius auratus  [in Japanese]

A new fibroblastic cell line was established from the caudal fin of the goldfish, Carassius auratus. The cells, designated CAF have been subcultured over 80 times since their initiation in August 1977. There was no difference in the growth rate between 10 and 20% fetal calf serum. At 5% fetal calf serum and 20% calf serum, the growth curves fell slightly lower than the 10 and 20% levels of fetal calf serum. CAF cells were cultured at various temperatures. The rate of cell growth increased with increasing temperature over the range of 16 to 33℃, whereas no cell growth was observed at 6℃. Plating efficiency of CAF cells was estimated to be 10%, when 2,000 or more cells per dish were inoculated at 26℃.