Cell types involved in allogeneic contact reactions of the solitary ascidian, Halocynthia roretzi

• Fuke, Masako

• 富家, 雅子

書誌事項

タイトル別名

• Cell Types Involved in Allogeneic Contact Reactions of the Solitary Ascidian, Halocynthia roretzi(Immunology) :

抄録

金沢大学理学部

The blood cells of a solitary ascidian, Halocynthia roretzi exhibit the allogeneic cellular reaction in vitro denoted as the "contact reaction"(Fuke, 1980). Nine cell types have been recognized in the blood and body fluid of H. roretzi (Fuke and Fukumoto, 1993). In the present study, the precise role of each cell type in allogeneic reactions is investigated in vitro. The vacuolated cells show devacuolation after contact with almost all other cell types from different reactive animals. These cells include hyaline amoebocytes, granular amoebocytes, macrogranular cells, small amoebocytes and giant cells (large basophilic cells), as well as vacuolated cells. The hyaline amoebocytes and small amoebocytes, which belong to the phagocyte series exhibit contact reactions with the cells of phagocyte series from other specimens of H. roretzi. They also show a contact reaction when in contact with granular amoebocytes. Therefore, almost all cell types have the materials responsible for individuality on their cell surface and can directly show the allogeneic cellular reactivity when they contact each other. To determine whether the contact reaction is involved in cell death, the loss of plasma membrane integrity was examined using fluorescent dyes. The number of cells showing uptake of ethidium bromide increased immediately after mixing of allogeneic cells. Almost the same cell types as those showing allogeneic behavior by light microscopy, as described above, exhibited loss of cell membrane integrity. These results are discussed in the context of immune systems in invertebrates and vertebrates.

収録刊行物

• Zoological science

  Zoological science 18 (2), 195-205, 2001-03-01

  Zoological Society of Japan = 日本動物学会

キーワード

• Animal Science and Zoology

詳細情報

• CRID: 1050845760872640256
• NII論文ID: 110003371919
• NII書誌ID: AA10545874
• ISSN: 02891824
• ISSN: 02890003
• DOI: 10.2108/zsj.18.195
• NDL書誌ID: 6408862
• Web Site: http://hdl.handle.net/2297/28983
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6408862
• Web Site: http://www.bioone.org/doi/pdf/10.2108/zsj.18.195
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10864063
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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