Fate of Orally Administered 15N-Labeled Polyamines in Rats Bearing Solid Tumors.

• Kobayashi Masaki

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University

• Xu Yong Ji

  Institute of Chemical & Molecular Technology, Qingdao University of Science and Technology

• Samejima Keijiro

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University

• Goda Hitomi

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University

• Niitsu Masaru

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University

• Takahashi Masakazu

  Department of Pathology, Sasaki Institute

• Hashimoto Yoshiyuki

  Kyoritsu College of Pharmacy

抄録

We studied absorption, distribution, metabolism, and excretion of polyamines (putrescine, spermidine, and spermine) in the gastrointestinal tract using ¹⁵N-labeled polyamines as tracers and ionspray ionization mass spectrometry (IS-MS). The relatively simple protocol using rats bearing solid tumors provided useful information. Three ¹⁵N-labeled polyamines that were simultaneously administered were absorbed equally from gastrointestinal tract, and distributed within tissues at various concentrations. The uptake of ¹⁵N-spermidine seemed preferential to that of ¹⁵N-spermine since the concentrations of ¹⁵N-spermidine in the liver and tumors were higher, whereas those of ¹⁵N-spermine were higher in the kidney, probably due to the excretion of excess extracellular spermine. Most of the absorbed ¹⁵N-putrescine seemed to be lost, suggesting blood and tissue diamine oxidase degradation. Concentrations of ¹⁵N-spermidine and ¹⁵N-spermine in the tumor were low. We also describe the findings from two rats that were administered with ¹⁵N-spermine. The tissue concentrations of ¹⁵N-spermine were unusually high, and significant levels of ¹⁵N-spermidine were derived from ¹⁵N-spermine in these animals.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 26 (3), 285-288, 2003

  公益社団法人 日本薬学会

キーワード

• ¹⁵N-labeled polyamine
• oral administration
• ionspray ionization mass spectrometry
• Donryu rat
• ascites hepatoma cell

詳細情報

• CRID: 1390001204628138880
• NII論文ID: 110003608418
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.26.285
• COI: 1:CAS:528:DC%2BD3sXjsVajur4%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 6458053
• PubMed: 12612433
• Web Site: http://id.ndl.go.jp/bib/6458053
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6458053
• Web Site: http://www.jstage.jst.go.jp/article/bpb/26/3/26_3_285/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003185797
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可