Effect of the Isocoulmarin Paepalantine on the Luminol and Lucigenin Amplified Chemiluminescence of Rat Neutrophils

DOI Web Site Web Site PubMed 被引用文献2件 参考文献37件
  • Kitagawa Rodrigo Rezende
    Instituto de Química de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho”
  • Raddi Maria Stella Gonçalves
    Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho”
  • Khalil Najeh Maissar
    Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho”
  • Vilegas Wagner
    Instituto de Química de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho”
  • Fonseca Luiz Marcos da
    Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho”

書誌事項

タイトル別名
  • Effect of the Isocoumarin Paepalantine on the Luminol and Lucigenin Amplified Chemiluminescence of Rat Neutrophils

この論文をさがす

抄録

Paepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naphto(2,3c)pyran-1-one), a natural isocoumarin isolated from the capitula of Paepalanthus bromelioides (Eriocaulaceae), was assessed for its effect on the respiratory burst (zymosan-stimulated luminol-enhanced chemiluminescence and PMA-stimulated lucigenin-enhanced chemiluminescence) of polymorphonuclear neutrophils in vitro. Special attention was devoted to establishing the IC50 for neutrophils. Paepalantine was able to decrease luminol and lucigenin chemiluminescence, reflecting an inhibitory effect on the respiratory burst, with an ED50 of 0.44±0.05 and 0.84±0.15 μg/ml, respectively. A cell-free system was performed with paepalantine on myeloperoxidase/H2O2 and myeloperoxidase/H2O2/Cl− systems. Paepalantine inhibited luminol oxidation in both systems. This inhibition was related to the interaction of paepalantine-myeloperoxidase and its scavenger effect on HOCl.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (37)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ