Neutralizing Antibody Evasion Ability of Adenovirus Vector Induced by the Bioconjugation of Methoxypolyethylene Glycol Succinimidyl Propionate (MPEG-SPA)

• Eto Yusuke

  Graduate School of Pharmaceutical Sciences, Osaka University

• Gao Jian-Qing

  Graduate School of Pharmaceutical Sciences, Osaka University Department of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University

• Sekiguchi Fumiko

  Graduate School of Pharmaceutical Sciences, Osaka University

• Kurachi Shinnosuke

  Graduate School of Pharmaceutical Sciences, Osaka University

• Katayama Kazufumi

  Graduate School of Pharmaceutical Sciences, Osaka University

• Mizuguchi Hiroyuki

  Division of Cellular and Gene Therapy Products, National Institute of Health Sciences, Japan

• Hayakawa Takao

  National Institute of Health Sciences, Japan

• Tsutsumi Yasuo

  Graduate School of Pharmaceutical Sciences, Osaka University

• Mayumi Tadanori

  Graduate School of Pharmaceutical Sciences, Osaka University

• Nakagawa Shinsaku

  Graduate School of Pharmaceutical Sciences, Osaka University

抄録

Although adenovirus vectors (Ad) which possesses high transduction efficiency are widely used for gene therapy in animal models, clinical use is very limited. One of the main reason is that nearly 80% of human beings possess anti-Ad antibodies. In this study, we tried to modify Ad with methoxypolyethylene glycol (MPEG) activated by succinimidyl propionate, and, the neutralizing antibody evasion ability of PEGylated Ad was evaluated. The results demonstrated that PEG-Ad showed stronger protection ability against anti-Ad neutralizing antibody compared to that with unmodified-Ad. Considering there are many people carrying neutralizing antibody against Ad and readministration of Ad was necessary for treating chronic diseases, this strategy, which was also applicable to other vectors, can be used for developing improved vectors.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 27 (6), 936-938, 2004

  公益社団法人 日本薬学会

キーワード

• adenovirus vector
• polyethylene glycol
• antibody
• gene therapy

詳細情報

• CRID: 1390001204626288000
• NII論文ID: 110003608890
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.27.936
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 6952580
• PubMed: 15187452
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6952580
• Web Site: http://www.jstage.jst.go.jp/article/bpb/27/6/27_6_936/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004283013
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可