Modulation of Corydalis tuber on Glycine-Induced Ion Current in Acutely Dissociated Rat Periaqueductal Gray Neurons(Pharmacology) CHEONG Byung-Shik CHOI Do-Young CHO Nam-Hun LEE Jae-Dong CHANG Hyun-Kyung SHIN Min-Chul SHIN Mal-Soon KIM Chang-Ju 公益社団法人日本薬学会 Biological & pharmaceutical bulletin 09186158 27 8 1207 1211 2004-08-01 Traditionally, Corydalis tuber has been used for the control of pain including headache, stomachache, and neuralgia. In the present study, modulation of the Corydalis tuber on glycine-activated ion current in the acutely dissociated periaqueductal gray (PAG) neurons was studied by a nystatin-perforated patch-clamp technique. High concentrations of Corydalis tuber elicited ion current, which was suppressed by strychnine application, while low concentrations of Corydalis tuber reduced glycine-induced ion current in the PAG neurons. Inhibitory action of Corydalis tuber on glycine-activated ion current was partially abolished by treatment with naltrexone, a non-selective opioid antagonist. Application of N-methylmalemide (NEM), a sulfhydryl alkylating agent, also partially abolished the inhibitory action of Corydalis tuber on glycine-activated ion current in the PAG neurons. These results suggest that the inhibitory effect of Corydalis tuber on glycine-activated ion current in the PAG neurons is one of the analgesic mechanisms of the Corydalis tuber. 2004-08-01 110003608946 AA10885497 7020939 ENG CJP NDL NII-ELS J-STAGE 3 25 Modulation of Corydalis tuber on Glycine-Induced Ion Current in Acutely Dissociated Rat Periaqueductal Gray Neurons(Pharmacology) CHEONG Byung-Shik CHOI Do-Young CHO Nam-Hun LEE Jae-Dong CHANG Hyun-Kyung SHIN Min-Chul SHIN Mal-Soon KIM Chang-Ju The Pharmaceutical Society of Japan Biological & pharmaceutical bulletin Traditionally, Corydalis tuber has been used for the control of pain including headache, stomachache, and neuralgia. In the present study, modulation of the Corydalis tuber on glycine-activated ion current in the acutely dissociated periaqueductal gray (PAG) neurons was studied by a nystatin-perforated patch-clamp technique. High concentrations of Corydalis tuber elicited ion current, which was suppressed by strychnine application, while low concentrations of Corydalis tuber reduced glycine-induced ion current in the PAG neurons. Inhibitory action of Corydalis tuber on glycine-activated ion current was partially abolished by treatment with naltrexone, a non-selective opioid antagonist. Application of N-methylmalemide (NEM), a sulfhydryl alkylating agent, also partially abolished the inhibitory action of Corydalis tuber on glycine-activated ion current in the PAG neurons. These results suggest that the inhibitory effect of Corydalis tuber on glycine-activated ion current in the PAG neurons is one of the analgesic mechanisms of the Corydalis tuber. CHEONG Byung-Shik Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University CHOI Do-Young Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University CHO Nam-Hun Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University LEE Jae-Dong Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University CHANG Hyun-Kyung Department of Physiology, College of Medicine, Kyung Hee University SHIN Min-Chul Department of Physiology, College of Medicine, Kyung Hee University SHIN Mal-Soon Department of Physiology, College of Medicine, Kyung Hee University KIM Chang-Ju Department of Physiology, College of Medicine, Kyung Hee University