The Combination of Rat Mast Cell and Rabbit Aortic Smooth Muscle Is the Simple Bioassay for the Screening of Anti-allergic Ingreditent from Methanolic Extract of Corydalis Tuber

• Saito Shin-ya

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Tanaka Masashi

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Matsunaga Kimihiro

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Li Yushan

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

• Ohizumi Yasushi

  Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

書誌事項

タイトル別名

• Combination of Rat Mast Cell and Rabbit Aortic Smooth Muscle Is the Simple Bioassay for the Screening of Anti allergic Ingreditent from Methanolic Extract of Corydalis Tuber

抄録

We have assessed the release of histamine from mast cells by smooth muscle contraction. 0.3 μg/ml compound 48/80 showed no effect on concentration–response relationship of histamine in rabbit aorta. Compound 48/80 induced release of histamine from rat mast cells. When aorta was stimulated by compound 48/80 in the presence of mast cells, contraction was evoked in concentration-dependent manner. This mast cell-dependent contraction was completely blocked by H₁ receptor antagonist, 1 μM diphenhydramine. When mast cells was treated with compound 48/80 inhibitor benzalkonium chloride, mast cell-dependent contraction was inhibited, although benzalkonium chloride itself showed no effect on concentration–response relationship of histamine in rabbit aorta. At high concentration of 10 μg/ml, benzalkonium chloride itself evoked histamine release from mast cells and indeed inhibitory effect of 10 μg/ml benzalkonium chloride on mast cell-dependent contraction was lower than that of 3 μg/ml. We have applied this bioassay to search anti-allergic ingredient from a total methanolic extract of Corydalis tuber (Corydalis turtschaninovii BESSER forma yanhusuo Y. H. CHOU et C. C. HSU). Successively, we have isolated five fractions. The fractions I—IV are identified to be corybulbine (1), tetrahydropalmatine (2), corydaline (3) and yuanhunine (4), respectively. Main component of fraction V is the mixture of 3 and canadine (5). Fractions II and V significantly inhibited mast cell-dependent contraction in rabbit aorta as well as inhibited histamine release from rat mast cells. Furthermore, fractions I, III and V inhibited histamine-induced contraction in rabbit aorta at non-competitive manner. From these results, combination of rat mast cells and rabbit aorta is good bioassay to search the anti-allergic ingredient, and we have obtained effective fractions from Corydalis tuber using this assay.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 27 (8), 1270-1274, 2004

  公益社団法人 日本薬学会

キーワード

• Corydalis tuber
• rat mast cell
• rabbit aorta
• anti-allergic ingredient

詳細情報

• CRID: 1390001204626503168
• NII論文ID: 110003608958
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.27.1270
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 7021155
• PubMed: 15305035
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7021155
• Web Site: http://www.jstage.jst.go.jp/article/bpb/27/8/27_8_1270/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005201308
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可