Reverse-phase HPLC Determination and Pharmacokinetic Study of Vanillic Acid in the Plasma of Rats Treated with the Traditional Chinese Medicinal Preparation Di-Gu-Pi Decoction

• LI Kang

  Shenyang Pharmaceutical University

• CHEN Xiaohui

  Shenyang Pharmaceutical University

• JIA Ying

  Shenyang Pharmaceutical University

• BI Kaishun

  Shenyang Pharmaceutical University

Abstract

  A sensitive, simple, and accurate method for the determination and pharmacokinetic study of vanillic acid in rat plasma was developed using reverse-phase HPLC with UV detection after oral administration of the traditional Chinese medicine preparation of the Di-Gu-Pi decoction. Plasma samples taken from rats were extracted with methanol. The constituent vanillic acid was separated on a C₁₈ stationary phase and a mobile phase of acetonitrile-water (15:85, v/v) (adjusted to pH 3.0 using phosphoric acid), with a UV detector setting at 260 nm. The validated HPLC method developed was used to determine the pharmacokinetic profile of vanillic acid in rat plasma after administration of the Di-Gu-Pi decoction.<br>

Journal

• YAKUGAKU ZASSHI

  YAKUGAKU ZASSHI 124 (7), 465-468, 2004-07-01

  The Pharmaceutical Society of Japan

Keywords

• Di-Gu-Pi decoction
• vanillic acid
• pharmacokinetics

Details

• CRID: 1390282681106158464
• NII Article ID: 110003614977
• NII Book ID: AN00284903
• DOI: 10.1248/yakushi.124.465
• ISSN: 13475231
• ISSN: 00316903
• NDL BIB ID: 7016667
• PubMed: 15235230
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7016667
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/124/7/124_7_465/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005128320
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed