Simultaneous Determination of Chlorogenic Acid, Forsythin and Arctiin in Chinese Traditional Medicines Preparation by Reversed Phase-HPLC

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Abstract

A reversed phase (RP)-HPLC method was established for simultaneous determination of ehlorogenic acid, arctiin and forsythin in Yinqiao Jiedu Granules, which was a commonly used Chinese herbal medical preparation for treatment of rheum ailments. The determination was based on a gradient elution (A : 1% acetic acid, pH = 3.0, B : methanol) on a C18 column and an automatic wavelength switching program, where 325 nm was used for ehlorogenic acid and 280 nm for arctiin and forsythin, respectively. Good linearities were obtained over the range of 2-200 mg・l^<-1> for the 3 objective compounds. The spike recoveries were within 96.0-97.9%.

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Codes

  • NII Article ID (NAID)
    110003615318
  • NII NACSIS-CAT ID (NCID)
    AA00602100
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    00092363
  • NDL Article ID
    6492378
  • NDL Source Classification
    ZS51(科学技術--薬学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-D167
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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