Simultaneous Determination of Chlorogenic Acid, Forsythin and Arctiin in Chinese Traditional Medicines Preparation by Reversed Phase-HPLC

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Abstract

A reversed phase (RP)-HPLC method was established for simultaneous determination of ehlorogenic acid, arctiin and forsythin in Yinqiao Jiedu Granules, which was a commonly used Chinese herbal medical preparation for treatment of rheum ailments. The determination was based on a gradient elution (A : 1% acetic acid, pH = 3.0, B : methanol) on a C18 column and an automatic wavelength switching program, where 325 nm was used for ehlorogenic acid and 280 nm for arctiin and forsythin, respectively. Good linearities were obtained over the range of 2-200 mg・l^<-1> for the 3 objective compounds. The spike recoveries were within 96.0-97.9%.

Journal

Chemical & pharmaceutical bulletin   [List of Volumes]

Chemical & pharmaceutical bulletin 51(4), 421-424, 2003-04-01  [Table of Contents]

The Pharmaceutical Society of Japan

References:  36

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Codes

  • NII Article ID (NAID) :
    110003615318
  • NII NACSIS-CAT ID (NCID) :
    AA00602100
  • Text Lang :
    ENG
  • Article Type :
    NOT
  • ISSN :
    00092363
  • NDL Article ID :
    6492378
  • NDL Source Classification :
    ZS51(科学技術--薬学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No. :
    Z53-D167
  • Databases :
    CJP  NDL  NII-ELS  J-STAGE