A New Fluorometric Assay for Human Serum Monoamine Oxidase

A new fluorometric method for the sensitive assay of human serum monoamine oxidase is presented. This is based on the determination of benzaldehyde produced from the substrate benzylamine under the optimal enzyme reaction conditions by means of the previously established fluorometric method for selective determination of aromatic aldehydes with 1,2-diaminonaphthalene sulfate. This method gives reliable results and is readily performed with a minimum amount of serum.