Application of High-performance Liquid Chromatography to the Isolation of Ginsenoside-Rf, -Rg_2,and -Rh_1 from a Crude Saponin Mixture of Ginseng
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The minor components of ginseng saponins, ginsenoside-Rf, -Rg_2,and -Rh_1,were isolated from the crude saponin fraction by a high-performance liquid chromatography (HPLC) procedure involving preparative HPLC on silica gel followed by semi-preparative HPLC on a column packing of Carbohydrate Analysis. This method was rapid and convenient.