3-(Difluoro-1,3,5-triazinyl)-1-(ethylthio)-2-n-propylbenz[f]isoindole as a Fluorescence Derivatization Reagent for Estrogens in High-Performance Liquid Chromatography

• FUJINO Hiroyuki

  Faculty of Pharmaceutical Sciences, Kumamoto University

• GOYA Shujiro

  Faculty of Pharmaceutical Sciences, Kumamoto University

Abstract

A new fluorogenic reagent, 3-(difluoro-1,3,5-triazinyl)-1-(ethylthio)-2-n-propylbenz[f]isoindole, which reacts with phenolic hydroxyl groups, has been synthesized. This reagent reacts with estrogens in aqueous alkali-acetone mixtures at room temperature for 5 s to form the corresponding fluorescent derivatives, which can be separated by reversed-phase chromatography on TSK gel ODS-120A using methanol as a mobile phase. The detection limit for estrone was 80 fmol for an injection volume of 10 μl.

Journal

• Chemical & pharmaceutical bulletin

  Chemical & pharmaceutical bulletin 37 (7), 1939-1940, 1989-07-25

  The Pharmaceutical Society of Japan

Keywords

• 3-(difluoro-1, 3, 5-triazinyl)-1-(ethylthio)-2-n-propylbenz[f]isoindole
• phenolic hydroxyl group
• estrogen
• fluorogenic reagent
• high-performance liquid chromatography

Details

• CRID: 1571698602414915712
• NII Article ID: 110003627897
• NII Book ID: AA00602100
• ISSN: 00092363
• Text Lang: en
• Data Source:
  • CiNii Articles