Studies on the chemical modification of monensin. II. Measurement of sodium ion pemeability of monensylamino acids using sodium-23 nuclear magnetic resonance spectroscopy.

• 中村 明人

• 永井 慎一

• 植田 泰誠

• 榊原 仁作

• 堀田 芳弘

• 竹谷 和視

抄録

A technique to assay Na⁺ ions in cells is presented. Intracellular and extracellular Na⁺ ions in a suspension of guinea pig erythrocytes were conveniently determined by using sodium-23 unclear magnetic resonance (²³Na-NMR), in combination with two anionic shift reagents : Dy(TTHA)^3- and Dy(PPPi)₂^7-. Monensin (1), monensylalanine (2b), monensylserine (2c), and monensylphenylalanine (2d) induced large increases of intracellular Na⁺ ion concentration ([Na_in]), while monensylglycine (2a), monensyltyrosine (2e), monensylaspartic acid (2f), and monensylglutamic acid (2g) showed slight increases. The values of initial increasing rate (V_i) of 2a-g were much smaller than that of 1. This fact was probably due to the lower lipophilicity of 2a-g than 1, because a good correlation was observed between V_i and Rm₅₀ values of 1 and 2a-g. This lower lipophilicity is a consequence of conformational differences between 1 and 2a-g.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 37 (9), 2330-2333, 1989

  公益社団法人 日本薬学会

キーワード

• monensin
• monensylamino acid
• sodium ion permeability
• intracellular sodium ion concentration
• ^<23>Na-NMR
• guinea pig blood
• shift reagent
• external reference
• Rm<SUB>50</SUB> value
• positive inotropic effect

詳細情報

• CRID: 1390001204166674944
• NII論文ID: 110003627992
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.37.2330
• ISSN: 13475223
• ISSN: 00092363
• PubMed: 2605678
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/37/9/37_9_2330/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可