Application of ion-pair high performance liquid chromatography for analysis of hyoscyamine and scopolamine in solanaceous crude drugs.

• 大島 俊幸

• 相楽 和彦

• 童 玉一

• 章 観徳

• 陳 毓亨

抄録

A new ion-pair high-performance liquid chromatographic method for the analysis of hyoscyamine and scopolamine in various kinds of crude drugs derived from solanaceous plants was evaluated using sodium dodecyl sulfate as a counter ion. A reversed-phase chromatographic system consisting of a chemically bonded ODS silica gel column with phosphate buffer (pH 2.5)-acetonitrile (65 : 35) containing 17.5mM sodium dodecyl sulfate as the mobile phase was used. Hyoscyamine and scopolamine in crude drugs derived from Scopolia, Atropa, Hyoscyamus and Datura species were separated from other compounds in the crude drugs and determined within 20 min after direct injection of the solution extracted with the mobile phase. The results for various kinds of samples are presented.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 37 (9), 2456-2458, 1989

  公益社団法人 日本薬学会

キーワード

• ion-pair high-performance liquid chromatography
• scopolamine
• hyoscyamine
• atropine
• Scopolia japonica
• Atropa belladonna
• Hyoscyamus niger
• Datura metel

詳細情報

• CRID: 1390001204167894400
• NII論文ID: 110003628017
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.37.2456
• ISSN: 13475223
• ISSN: 00092363
• PubMed: 2605691
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/37/9/37_9_2456/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1991073389
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可