An Enzyme-Linked Immunosorbent Assay for the Detection of Plasminogen Activator-Induced Fribrin Clot Lysis in a Circulating Plasma System

A technically easy and sensitive method for the evaluation of the fibrinolytic potency of plasminogen activator in a circulating plasma system was developed. A circulating apparatus was prepared by connecting silicone tubes with two separate chambers. Human plasma and plasminogen activator were allowed to circulate from one chamber through a peristaltic pump into another one which contained biotin-labeled fibrin clot. The extent of fibrinolysis was evaluated on streptavidin-coated microtiter plates by measuring the amount of the avidin-bound fibrin degradation products with peroxidase-linked antibody. The detection limit of the fibrinolytic products was 1.5ng/ml. The present method dose not require any specialized equipment and is also applicable to a low-cost routine measurement of the thrombus selectivity of plasminogen activator.