Comparison of Flanking Regions of the 5S Ribosomal Ribonucleic Acid Genes in Leptospira biflexa and Leptospira interrogans

One of the genes encoding the 5S ribosomal ribonucleic acid (rRNA) for the Leptospira biflexa strain Patoc I was isolated and sequenced. The physical maps of the 5S rRNA genes in Leptospira were constructed. The strains of Leptospira biflexa had two genes on their chromosome; these two 5S rRNA genes were located several kb apart and sequences flanking these genes were divergent. In contrast to saprophytic leptospires, maps in parasitic leptopires that had only one gene for 5S rRNA on their genome were highly conserved and the physical maps of the genes in almost all strains were similar.