Inhibitory Effects of Pentagalloylglucose on Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase of Photobacterium phosphoreum.

• 足立 博一

• 古西 清司

• 堀越 勇

抄録

Inhibitory effects of pure pentagalloylglucose (1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucose) on purified nicotinamide adenine dinucleotide (NADH) dehydrogenase of the respiratory chain of Photobacterium phosphoreum were investigated. Pentagalloylglucose inhibited the NADH-ubiquinone-1, NADH-menadione, and NADH-2, 6-dichlorophenolindophenol oxidoreductase activities with less than 150nM of a 50% inhibition concentration (IC₅₀), but hardly inhibited NADH-ferricyanide and NADH-cytochrome c oxidoreductase activities with at least less than 0.5μM of pentagalloylglucose.Pentagalloylglucose inhibited noncompetitively the NADH dehydrogenase pf P. phosphoreum, which belongs to NADH dehydrogenase II (NADH dh II), and its inhibitor constant (K_i) to NADH-ubiquinone-1 oxidoreductase activity was estimated as 30nM of pentagalloylglucose. Therefore, pentagalloylglucose is the potent inhibitor of NADH dh II, more than flavone (IC₅₀=150μM for NADH-ubiquinone-1 oxidoreductase activity) which has previously been reported as an inhibitor for NADH dh II.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 40 (3), 718-720, 1992

  公益社団法人 日本薬学会

キーワード

• pentagalloylglucose
• respiratory chain
• nicotinamide adenine dinucleotide dehydrogenase
• Photobacterium phosphoreum

詳細情報

• CRID: 1390001204159519616
• NII論文ID: 110003629846
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.40.718
• ISSN: 13475223
• ISSN: 00092363
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/40/3/40_3_718/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可