Glycyrrhetylamino acids: Synthesis and application to enzyme immunoassay for glycyrrhetic acid.
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Glycyrrhetylamino acids (5a-c) were prepared by the condensation of glycyrrhetic acid (GA) with amino acids (glycine, γ-aminobutyric acid, and ε-aminohexanoic acid), which were selected for use as chemical bridges between the hapten and carrier protein in an enzyme immunoassay (EIA) for GA. The condensation was carried out in the presence of dicyclohexylcarbodiimide (method A), diphenyl phosphorazidate (method B) or diethyl phosphorocyanidate (method C), and method C gave the desired glycyrrhetylamino acids (5a-c) in the best yields. β-Galactosidase was used as the labeled enzyme and was conjugated with GA by the N-hydroxysuccinimide ester method. Separation of bound and free fractions was performed by a double antibody method using a goat antiserum to rabbit IgG. 7-β-D-Galactopyranosyloxy-4-methylcoumarin was used as substrate for the fluorometric assay of β-galactosidase activity. A satisfactory standard curve for GA was obtained in the range of 2.5-250 ng/ml.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 29 (6), 1533-1538, 1981
公益社団法人 日本薬学会
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詳細情報
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- CRID
- 1390001204163626752
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- NII論文ID
- 110003633860
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可