Fluorimetric assay for catechol-O-methyltransferase.

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A sensitive fluorimetric method for the assay of catechol-O-methyltransferase in a sample solution prepared from rat liver is described. Vanillin formed from the substrate, 3, 4-dihydroxybenzaldehyde, under the optimal conditions for the enzyme reaction, is determined after the removal of unreacted substrate by adsorption on alumina. Vanillin is measured by the established fluorimetric method for the determination of aromatic aldehydes with 2, 2'-dithiobis (1-aminonaphthalene). The limit of determination for vanillin formed is 300 pmol. The method is readily performed with good precision and is suitable for the rapid assay of catechol-O-methyltransferase in a small amount of sample solution.

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