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Hog kidney mutarotase was separated into four forms (types I-IV) by DEAE-cellulose column chromatography. The most abundant form (type II) was purified to homogeneity as judged by polyacrylamide disc gel electrophoresis. The physico-chemical properties of the pure type II enzyme were as follows : molecular weight, 41000 ; isoelectric point, pH 5.48 ; Km for α-D-glucose at pH 7.4 and at 25°, 19 mM ; optimum pH, 6.5-7.5 ; optimum temperature, 30°. The enzyme activity was greatly reduced at acid pH below 6.0. The enzyme lost little activity on storage for at least 130 days at 4°, whereas about 12% of the activity was lost during the same period at -20°. When the enzyme was heated for 10 min at 59°, the activity was completely lost.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 29 (6), 1702-1707, 1981
公益社団法人 日本薬学会
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詳細情報
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- CRID
- 1390001204162675072
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- NII論文ID
- 110003633882
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- PubMed
- 7296720
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- PubMed
- CiNii Articles
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- 使用不可