Studies on heterogeneous components of hog pancreatic kallikrein. II. Preliminary probe of the varied structures of carbohydrate chain of hog pancreatic kallikrein.
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Highly purified hog pancreatic kallikreins A and B from the autolysed hog pancreas were digested with pronase and subjected to hydrazinolysis to release asparagine-linked sugar chains. The oligosaccharides released were separated into fractions based on the acidities due to the contents of neuraminic acid residues. Out of these oligosaccharides, fractions having no neuraminic acid obtained from both A and B were further separated according to the molecular size by the 2 times repeated gel filtrations and their carbohydrate contents were analysed. Thus varied primary structures of carbohydrate chains-namely, different number of neuraminic acid residues being bound, straight and branched oligosaccharide chains, and the high mannose containing and the complex carbohydrates containing structures-of these asparagine-linked oligosaccharides being consisted parts of hog pancreatic kallikreins were observed.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 29 (6), 1785-1788, 1981
公益社団法人 日本薬学会
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詳細情報
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- CRID
- 1390001204163678720
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- NII論文ID
- 110003633902
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- PubMed
- 6913438
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可