Enzyme immunoassay for cuprozinc-superoxide dismutase in serum and urine

• NISHIMURA N

  Gifu College of Pharmachy

• ITO YOSHIMASA

  Gifu College of Pharmachy

• ADACHI TETSUO

  Gifu College of Pharmachy

• HIRANO KAZUYUKI

  Gifu College of Pharmachy

• SUGIURA MAMORU

  Gifu College of Pharmachy

• SAWAKI SHUNJI

  First Department of Internal Medicine, Aichi Medical University

抄録

A highly sensitive enzyme immunoassay system for the determination of cuprozinc-superoxide dismutase (Cu, Zn-SOD) in serum and urine by using β-galactosidase as a labeling enzyme is reported. This assay had a greater sensitivity than that of previously reported radioimmunoassay methods and could measure from 0.05 to 10.0 ng of Cu, Zn-SOD with good reproducibility. Coefficients of variation for this enzyme immunoassay were 2.8-8.3% within a day and 5.6-16.1% between days. The average recoveries were 96-103% from sera and 96-105% from urines, respectively. Using this enzyme immunoassay, serum Cu, Zn-SOD concentrations increased significantly in patients with renal diseases and were slightly elevated in patients with liver diseases. Urinary Cu, Zn-SOD appeared to be the highest in all renal diseases. By immunofluorescent staining, Cu, Zn-SOD was located in the thickened portions of the glomerular basement membrane and proliferating mesangial cells of the kidney tissue from a patient with membranous glomerulonephritis.

収録刊行物

• J Pharm Dyn

  J Pharm Dyn 5 394-402, 1982

  公益社団法人日本薬学会

キーワード

• enzyme immunoassay for cuprozinc-superoxide dismutase
• superoxide dismutase in serum and urine
• superoxide dismutase in kidney
• renal disease
• oxygen toxicity

詳細情報

• CRID: 1572824502208446464
• NII論文ID: 110003636376
• NII書誌ID: AA00704585
• ISSN: 0386846X
• Web Site: https://search.jamas.or.jp/link/ui/1983030032
• 本文言語コード: en
• データソース種別:
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